Sequencing |
|
|---|---|
| Number of Reads | 250,000 |
| Valid Barcodes | 84.76% |
| Sequencing Saturation | 28.90% | Q30 Bases in CB+UMI | 73.19% |
| GC Content | 42.95% |
| Q20 Bases in RNA Read | 98.05% |
| Q30 Bases in RNA Read | 94.97% |
| Fraction Reads in Spots Under Tissue | 95.51% |
|---|---|
| Mean Reads Under Tissue per Spot | 55 |
| Median UMI Counts per Spot | 42 |
| Median Genes per Spot | 39 |
| Total Genes Detected | 10,297 |
Mapping |
|
|---|---|
| Reads Mapped to Genome | 90.25% |
| Reads Mapped Confidently to Genome | 70.10% |
| Reads Mapped Confidently to Intergenic Regions | 7.46% |
| Reads Mapped Confidently to Intronic Regions | 7.03% |
| Reads Mapped Confidently to Exonic Regions | 55.60% |
Sample |
|
|---|---|
| Sample ID | demo |
| Sample Description | |
| Chemistry | Spatial Gene 3' v1 |
| Include introns | False |
| Transcriptome | mm10_gencodeM13 |
| Pipeline Version | DynamicST-1.0.2 |
UMIs Detected
Clustering
Sequencing Saturation
Median Genes per Spot